Background: Antibiotics are the most effective drugs against microbial infections, but slowly lose their efficacy to microorganisms due to resistance. So, nowadays, research related to finding new compounds from nature to treat infectious diseases has become important. Mussaenda frondosa L. is a tropical plant native to Indonesia. Traditionally, Mussaenda frondosa L. has been used for the treatment of various diseases, such as headaches and jaundice. The literature study of the antibacterial activity of Mussaenda frondosa L. is still very limited, so research needs to be done regarding the antibacterial activity.
Objective: to determine whether there is antibacterial activity from Mussaenda frondosa L. petal fraction. And whether there is significant antibacterial activity when it is compared to chloramphenicol as positive control antibiotic.
Method: The methodology of this research starts with sampling, sample identification, extraction, and fractionation. Screening for antibacterial activity was carried out by the agar diffusion method.
Results: The most potential antibacterial activity was ethyl acetate fraction with an inhibitory diameter of 5.6 - 8.3 mm against 12 test pathogenic bacteria, except Streptococcus mutans ATCC 25175. The n-hexane fraction showed antibacterial activity against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and Vibrio cholerae inaba with an inhibitory diameter of 6.0 - 7.5 mm. Meanwhile, the n-butanol fraction has only antibacterial activity against Staphylococcus aureus ATCC 25923 with a 7.5 mm inhibition diameter.
Conclusion: the most potential fraction that had antibacterial activity was ethyl acetate fraction, by inhibiting 12 test pathogenic bacterias, followed by n-hexane fraction which inhibits 3 bacterias.
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