Sambiloto leaf (Andrographis paniculata) and kemangi leaf (Ocimum sanctum) are alternatives that can be used in medicine as antibacterial. Staphylococcus aureus is a pathogen for the human body that can be treated with antibiotics. However, antibiotic resistance can occur if its use is not appropriate. So, alternative treatments with plants are used as essential therapeutic ingredients that have the potential as drugs. The purpose was to identify and analyze the antibacterial combination of sambiloto leaf extract and kemangi against the growth of Staphylococcus aureus bacteria. The extract was obtained by maceration method using 96% ethanol and tested for antibacterial activity using the diffusion method with various concentrations of 6.25% to 100% and tested at combination concentrations (50%:50%), (75%:25%), and (25%:75%). The concentration of sambiloto leaf at 80% and kemangi leaf at 6.25% were the outcomes of the optimal inhibitory zone generated in the extract. In the combination test, a concentration of 75%: 25% with an average diameter of 26.80. The p-value of the One-Way ANOVA test is 0.001, be concluded that each extract concentration affects bacterial growth. The conclusion is that the extracts of sambiloto leaves and kemangi were able to inhibit the growth of Staphylococcus aureus bacteria.
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