Jeruk sambal (Citrus microcarpa Bunge) is a plant that is widely known by the people of Kalimantan Barat which has the potential to contain various secondary metabolites. The purpose of this study was to determine the content of secondary metabolites contained in the juice of Jeruk sambal from Desa Kalimas, Kalimantan Barat. The study began with making Jeruk sambal juice by washing the Jeruk sambal fruit under running water and drying it. Then the Jeruk sambal fruit was cut into two parts, squeezed manually using sterile gloves, and filtered twice using a plastic filter and sterile filter paper into a glass bottle. Phytochemical screening of Jeruk sambal juice was carried out qualitatively. The results of phytochemical screening showed that Jeruk sambal juice contains alkaloids, flavonoids, saponins, tannins, and saponins. The highest secondary metabolite content in Jeruk sambal juice is saponins (+++). Meanwhile, the test of steroids and terpenoids was found to be negative. The conclusion is that Jeruk sambal juice contains alkaloids, flavonoids, saponins, tannins, and saponins.
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