Anti-Inflamatory Activity and Cyclooxygenase-2 Inhibition of ethanol extract from Malur Leaves (Brucea javanica (L.) Merr)

Abstract

Inflammation is a normal protective response to tissue damage mediated by Cyclooxigenase-2 (COX-2) enzyme. This study aims to determine the anti-inflammatory activity ethanol extract of (Brucea javanica (L.) Merr.) and its inhibitory effect on COX-2 enzyme. The animals used in this study were male white rats which were divided into 5 groups, dose250 mg/kg BB, dose 500 mg/kg BB, comparative control (Celecoxib), positve control and negative controls. Determining of anti-inflammatory activity was carried out by inducing the soles of the Rats with carragen and then measuring the edema volume using a pletismometer and measuring COX-2 inhibiton using a microplate Reader. The results showed that the ethanol extract of malur leaves doses 250 mg/kg BB and 500 mg/kg BB had significant anti-inflammatory activity (p <0.05). The dose 500 mg/kg BB has a significant inhibitory effect on COX-2 (p <0.05), but dose 250 mg/kg BB does not have significant inhibitory effect on COX-2. It can be concluded that the ethanol extract of malur leaves has anti-inflammatory effects at doses 250 mg/kg BB and 500 mg/kg BB and inhibitory effect against COX-2 at a dose 500 mg/kg BB.