Citarum river pollution by fecal Coliform (Escherichia coli) is relatively high. Based on the results of monitoring in 2010 showed that the amount of E. coli in several areas drained by the Citarum river exceeded the Water Quality Limit (BMA), which was >1.000/100 ml. Citarum river irrigation is a source of raw water for one of the IPAM in Karawang, which is the source for most of the fulfillment of clean water for daily needs. E. coli can infect through water contamination so this condition can trigger the spread of the disease in the community. Diseases caused by bacterial infections are often treated with antibiotics. The use of antibiotics has increased tremendously in the last 5 decades, resulting in selective solid pressure and potentially triggering resistance in bacteria. This study aims to isolate E. coli and test its resistance to ampicillin and ceftriaxone antibiotics. The methods used in this study include presumptive test, confirmation test, Gram staining, and resistance testing using the Kirby-Bauer method, with the standard used in categorization referring to CLSI 2020. The results showed that E. coli isolates were still sensitive to ampicillin and ceftriaxone antibiotics with an inhibition zone of 26,16 mm and 33,27 mm.
Alkhyat, S. H., & Al.Maqtari, M. A. (2014). Prevalence of Microorganisms isolates from Urinary Tract Infections at Some Hospitals in Sana’a City, Yemen. International Journal of Current Microbiology and Applied Sciences, 3(6), 876–885.
Andriani, F., & Ariesyady, herto dwi. (2013). Microbiological Source Tracking Bakteri Escherichia coli dengan Metode Antibiotic Resistance Analysis di Sungai Cikapundung. Jurnal Teknik Lingkungan, 19(2), 170–176.
Arini, L. D. D., & Wulandari, R. M. (2017). Kontaminasi Bakteri Coliform Pada Saus Siomai dari Pedagang Area Kampus di Surakarta. BIOMEDIKA, 10(2), 31–46.
Astuti, D., & Arfania, M. (2018). Analisis Penggunaan Antibiotika Dengan Metoda ATC/DDD Di Rumah Sakit Swasta Kab Karawang. Pharma Xplore : Jurnal Sains Dan Ilmu Farmasi, 3(2), 194–202.
Astuti, D., & Nurhayati, Y. (2019). Evaluasi Kualitatif Penggunaan Antibiotik Pada Pasien Anak Dengan Metode Gyssens Di RSUD Karawang. Pharma Xplore : Jurnal Sains Dan Ilmu Farmasi, 4(1), 297–302.
Aulya, W., Fadhliani, & Mardina, V. (2020). Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method. Serambi Journal of Agricultural Technology (SJAT), 2(2), 64–72.
Baehaqi, K. Y., Putriningsih, P. A. S., & Suardana, I. W. (2015). Isolasi dan Identifikasi Escherichia Coli O157:H7 Dada Sapi Bali di Abiansemal, Badung, Bali. Indonesia Medicus Veterinus, 4(3), 267–278.
Catalanotti, C., Yang, W., C, M., & R, A. (2013). Fermentation Metabolism and its Evolution in Algae. Frontiers in Plant Science, 4(150), 1–17.
CLSI. (2020). Performance Standards for Antimicrobial Susceptibility Testing (30th ed.). Clinical and Laboratory Standards Institute.
Direktorat Pengembangan Kawasan Permukiman. (2017). Rencana Program Investasi (Infrastruktur) Jangka Menengah (RPIJM).
Dirga, Khairunnisa, S. M., Akhmad, A. D., Setyawan, I. A., & Pratama, A. (2021). Evaluasi Penggunaan Antibiotik Pada Pasien Rawat Inap Di Bangsal Penyakit Dalam RSUD. Dr. H. Abdul Moeloek Provinsi Lampung. Jurnal Kefarmasian Indonesia, 11(1), 65–75.
Fitriah, Mappiratu, & Prismawiryanti. (2017). Uji Aktivitas Antibakteri Ekstrak Daun Tanaman Johar (Cassia siamea Lamk.) dari Beberapa Tingkat Kepolaran Pelarut. Kovalen, 3(3), 242–251.
G, W., & JC, C. (2019). Long-term Antibiotics For Preventing Recurrent Urinary Tract Infection In Children. Cochrane Database System, 4(4), 1–57.
Gallagher, J. C., & MacDougall, C. (2016). Antibiotics Simplified (4th ed.). Jones & Bartlett Learning.
Gholamhassan Shahbazi, Rezaee, M. A., Nikkhahi, F., Ebrahimzadeh, S., Hemmati, F., Namarvar, B. B., & Gholizadeh, P. (2021). Characteristics of diarrheagenic Escherichia coli pathotypes among children under the age of 10 years with acute diarrhea. Gene Reports, 25(101318).
Guentzel, M. N. (1996). Escherichia, Klebsiella, Enterobacter, Serratia, Citrobacter, and Proteus. In S. Baron (Ed.), Medical Microbiology (4th ed.). Galveston (TX) : University of Texas Medical Branch at Galveston.
Hamidah, M. N., Rianingsih, L., & Romadhon. (2019). Aktivitas Antibakteri Isolat Bakteri Asam Laktat dari Peda dengan Jenis Ikan Berbeda Terhadap E.coli dan S.aureus. Jurnal Ilmu Dan Teknologi Perikanan, 1(2), 11–21.
Hasanah, F. (2018). Gambaran Penggunaan Antibiotik Pada Penderita Diare Akut Anak Rawat Jalan Di UPTD Puskesmas Lhok Bengkuang Kecamatan Tapaktuan. Jurnal Saintika, 18(1), 19–23.
Hijriani, H., Agustini, A., & Karnila, A. (2020). Pengetahuan Perilaku Hidup Bersih Sehat (PHBS) Pada Anak Dengan Diare Di Rumah Sakit Umum Kelas B Kabupaten Karawang. Jurnal Health Sains, 1(5), 1–7.
Jamilatun, & Aminah. (2016). Isolasi dan Identifikasi Escherichia coli pada Air Wudhu di Masjid yang Berada di Kota Tangerang. Jurnal Medikes, 3(1), 81–90.
Jarvis, T. R., Chan, L., & Gottlieb, T. (2014). Assessment and Management of Lower Urinary Tract Infection in Adults. Australian Prescriber, 37(1), 7–9.
Kartikasari, A. M., Hamid, I. S., Elziyad, M. T., Damayanti, R., Fikri, F., & Praja, R. N. (2019). Isolasi dan Identifikasi Bakteri Escherichia coli Kontaminan pada Daging Ayam Broiler di Rumah Potong Ayam Kabupaten Lamongan. Jurnal Medik Veteriner, 2(1), 66–71.
Lal, A., & Cheeptham, N. (2007). Eosin-Methylene Blue Agar Plates Protocol.
Lee, D. S., Lee, S.-J., & Choe, H.-S. (2018). Community-Acquired Urinary Tract Infection by Escherichia coli in the Era of Antibiotic Resistance. BioMed Reasearch International, 2018, 1–14.
Marganingrum, D., Roosmin, D., Pradono, & Sabar, A. (2013). Diferesiasi Sumber Pencemar Sungai Menggunakan Pendekatan Metode Indeks Pencemar (IP) (Studi Kasus : Hulu DAS Citarum). RISET : Geologi Dan Pertambangan, 23(1), 37–48.
Masood, S. H., & Aslam, N. (2010). In Vitro Susceptibility Test of Different Clinical Isolates Against Ceftriaxone. Oman Medical Journal, 25(3), 199–202.
Mohammed, S., Ahmed, M., & Karem, K. (2014). Incidence of Multi-Drug Resistant Escherichia coli Isolates from Blood and Urine in Kerbala, Iraq. Journal of Kerbala University, 12(4), 222–227.
Mubarak, Z., Chismirina, S., & Daulay, hafizah humairah. (2016). Aktivitas Antibakteri Ekstrak Propolis Alami dari Sarang Lebah Terhadap Pertumbuhan Enterococcus faecalis. Journal of Syiah Kuala Dentistry Society, 1(2), 175–186.
Myataza, A., Ogbomoede, A., Uche, E., Nontongana, N., & Ifeanyi, A. (2017). Incidence and Antimicrobial Susceptibility of Escherichia coli O157:H7 Isolates Recovered from Dairy Farms in Amathole District Municipality, Eastern Cape, South Africa. Asian Pacific Journal of Tropical Disease, 7(12), 765–770.
Nurjannah, L., & Novita, D. A. (2018). Uji Bakteri Coliform dan Escherichia coli pada Air Minum Isi Ulang dan Air Sumur di Kabupaten Cirebon. Jurnal Ilmu Alam Indonesia, 1(1), 60–68.
Ouchenir, L., Renaud, C., Khan, S., Bitnun, A., Boisvert, A.-A., McDonald, J., Bowes, J., Brophy, J., Barton, M., Ting, J., Hawkes, M., Roberts, A., & Robinson, J. L. (2017). The Epidemiology, Management, and Outcomes of Bacterial Meningitis in Infants. Pediatrics, 140(1), 1–8.
Percival, S. L., & Williams, D. W. (2014). Escherichia coli. In Microbiology of Waterborne Diseases (2nd ed., pp. 89–117).
Presiden RI. (2005). Peraturan Pemerintah Republik Indonesia Nomor 16 Tahun 2005 Tentang Pengembangan Sistem Penyediaan Air Minum.
Rahayu, W. P., Nurjanah, S., & Komalasari, E. (2018). Escherichia coli : Patogenitas, Analisis Dan Kajian Risiko. IPB Press.
Restina, D., Ramadhian, M. R., Soleha, T. U., & Warganegara, E. (2019). Identifikasi Bakteri Escherichia coli pada Air PDAM dan Air Sumur di Kelurahan Gedong Air Bandar Lampung. Jurnal Agromedicine, 6(1), 58–62.
Rosmania, & Yanti, F. (2020). Perhitungan Jumlah Bakteri di Laboratorium Mikrobiologi Menggunakan Pengembangan Metode Spektrofotometri. Jurnal Penelitian Sains, 22(2), 76–86.
Saeed, A., Hamid, S. A., Bayoumi, M., Shanan, S., Alouffi, S., Alharbi, S. A., Alshammari, F. D., & Abd, H. (2017). Elevated Antibiotic Resistance of Sudanese Urinary Tract Infection Bacteria. EXCLI Journal, 16, 1073–1080.
Sari, R., & Apridamayanti, P. (2014). Cemaran Bakteri Escherichia coli dalam Beberapa Makanan Laut yang Beredar di Pasar Tradisional Kota Pontianak. Kartika Jurnal Ilmiah Farmasi, 2(2), 14–19.
Sasongko, H. (2014). Uji Resistensi Bakteri Escherichia Coli Dari Sungai Boyong Kabupaten Sleman Terhadap Antibiotik Amoksisilin, Kloramfenikol, Sulfametoxasol Dan Streptomisin. Jurnal BIOEDUTIKA, 2(1), 25–29.
Sholih, M. G., Sudarjat, H., & Saula, lely sulfiani. (2019). Gambaran Penggunaan Antibiotik Berdasarkan Metode ATC/DDD Dan DU 90% Di Salah Satu PUSKESMAS Karawang. Health Science Growth Journal, 4(1), 31–37.
Siswandono. (2016). Kimia Medisinal (2nd ed.). Airlangga University Press.
Soleha, M. (2013). Detection of attaching and effacing virulence gene of E. coli. Health Science Journal of Indonesia, 4(1), 41–46.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.