Tilapia (Oreochromis niloticus) is a freshwater fish that has the highest production volume in Indonesia. Tilapia fish is exported in the form of fresh whole, frozen whole, fresh filet frozen filet. Collagen is one of the many protein derivatives found in bones. This study aims to determine whether tilapia bone collagen can be formulated in the form of a cream which in certain concentrations can provide an anti-aging effect and not irritate the skin. This study used an experimental method, using tilapia (Oreochromis niloticus) bones as test material. The stages of this study included sampling, determination of animals, isolation of collagen from tilapia bones, testing the characteristics of collagen with infrared spectrophotometry, selecting the basic cream formula, determining the modified cream formula, making tilapia bone collagen cream with concentrations of 1%, 1.5%, 2.5% and 3.5% and evaluation of cream preparations and anti-aging effectiveness tests using the Skin analyzer checker (aramo®) where the results were statistically tested using the ANOVA test with the SPSS 26 Free trial. The results showed that tilapia bone collagen (Oreochromis niloticus) can be formulated into cream preparations, is a homogeneous and stable preparation with an oil-in-water (O/A) emulsion type, with a pH range immediately after being made 6.1-6.5 and after cycling test 6.0-6.4. Tilapia fish bone collagen cream (Oreochromis niloticus) with a concentration of 3.5% (F4) showed the best anti-aging effectiveness in terms of providing moisture of 50.2%, included in the "moist" category with a percent recovery of 24.7%, more higher compared to the comparison cream which provides moisture of 47.0% including the "moisturizing" category with a recovery percentage of 16.14%, 21.5% reduction in pore size, 22.1% reduction in the number of blemishes and 22.2% reduction in the number of wrinkles . The results of the probability statistical test were smaller than (p<0.05) statistically showing a significant difference with the comparison cream. All preparations of tilapia bone collagen cream (Oreochromis niloticus) do not irritate the skin.
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