The research has been regarding of method validation and determination S-allyl cisteine coumpound from solo black garlic extract by high perfomance liquid chromatography. The stationary phase used is ODS C-18 and the mobile phase is acetonitril : aquabidest (50:50 v/v) at a flow rate of 1,0 ml/min and detected at wavelength 195 nm for S-allyl cysteine. Before determination should be qualitative test determinant a maximum wavelength of sample. Standard solution S-allyl cysteine (SAC) with concentration 50, 100, 150, and 200 ppm wich is measured at a maximum wavelength of 195 nm, the value of regression line equation is y = 4,818x + 46,5 with the value of the correlation coefficient is (r) = 0,998. Obtained result sample wavelength 194 nm near to maximum wavelength of standard solution. The result of determination solo black garlic extract show for solo black garlic extract contain S-allyl cysteine 52,055 % with category high antioxidant activity 50-90 %. Based on the result it can be concluded that a HPLC is valid and the sample solo black garlic still show high S-allyl cysteine coumpound.
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